One irinotecan molecule stacks against the base pairs flanking the topoisomerase-induced cleavage site and poisons ( inactivates ) the topoisomerase 1 enzyme.
2.
The Barton reaction commences with a photochemically induced cleavage of the nitrite O-N bond, typically using a high pressure mercury lamp.
3.
One irinotecan or SN-38 molecule stacks against the base pairs flanking the topoisomerase-induced cleavage site and poisons ( inactivates ) the TOP1 enzyme.
4.
One irinotecan-derived SN-38 molecule stacks against the base pairs flanking the topoisomerase-induced cleavage site and poisons ( inactivates ) the topoisomerase 1 enzyme.
5.
RAG enzymes work as a multi-subunit complex to induce cleavage of a single double stranded DNA ( dsDNA ) molecule between the antigen receptor coding segment and a flanking recombination signal sequence ( RSS ).
6.
The most well-studied outcome of the RNAi is post-transcriptional gene silencing, which occurs when the guide strand pairs with a complementary sequence in a messenger RNA molecule and induces cleavage by Argonaute, that lies in the core of RISC.
7.
The microRNAs thus generated are short RNA molecules that regulate a wide variety of other genes by interacting with the RNA-induced silencing complex ( RISC ) to induce cleavage of complementary messenger RNA ( mRNA ) as part of the RNA interference pathway.
8.
In contrast, animal miRNAs are able to recognize their target mRNAs by using as little as 6 8 nucleotides ( the seed region ) at the 5'end of the miRNA, which is not enough pairing to induce cleavage of the target mRNAs.
9.
The most well-studied outcome is post-transcriptional gene silencing, which occurs when the guide strand pairs with a complementary sequence in a messenger RNA molecule and induces cleavage by Argonaute 2 ( Ago2 ), the catalytic component of the RISC complex.
10.
VR-RNA regulates the toxin colA ( collagenase ) by base-pairing to colA mRNA, the 3'end of VR-RNA base-pairs to a region in the 5'UTR of the colA mRNA . This induces cleavage of the colA mRNA in the 5'UTR and leads to stabilisation of the mRNA and increased translation.
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